THE PHYTOCHEMICAL CONSTITUENTS OF METHANOLIC EXTRACT OF OCIMUM GRATISSIMUM

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THE PHYTOCHEMICAL CONSTITUENTS OF METHANOLIC EXTRACT OF OCIMUM GRATISSIMUM

CHAPTER ONE

1.0     Introduction

1.1     Aim of Study

1.2     Literature Review

1.3     Phytochemicals

1.3.1  Alkaloids

1.3.2  Flavonoids

1.3.2.1 Antioxidant activities

1.3.2.2 Antimicrobial activities

1.3.2.3 Antibacterial activities

1.3.2.3 Antiatherosclerotic effects

1.3.3  Saponins

1.3.4  Tannins

CHAPTER TWO

2.0     Materials and Methods

2.1     Materials Used

2.2     Collection and Identification of samples

2.3     Sample preparation

2.3.1  Drying of the leaves

2.3.2      Pulverisation of the leaves

2.3.3      Extraction Procedure

2.3.4      Filtration Process

2.3.5      Concentration Process

2.4        Phytochemical Analysis

2.4.1   Oualitative Analysis

2.4.1.1 Test for Alkaloids

2.4.1.2 Test for Phytosterol

2.4.1.3 Test for Flavonoids

2.4.1.4 Test for Saponin

2.4.1.5 Test for Phenol and Tannin

2.4.2     Ouantitative Analysis

2.4.2.1 Determination of flavonoid content

2.4.2.2 Determination of tannins content

2.4.2.3 Determination of Saponins content

2.4.2.4 Determination of flavonoid content

2.4.2.5 Determination of Phenolic content

2.4.2.6 Determination of Reducing Power

CHAPTER THREE

3.0     Results

CHAPTER FOUR

4.0     Discussion and Conclusion

4.1     Discussion

4.2     Conclusion

References

LIST OF TABLES

Table 1 – Showing the qualitative analysis results of phytochemical constituents of Ocimum grattissimum                                                    

Table 2 – Showing the quantitative analysis of phytochemical constituents of Ocimum grattissimum 

Table 3 – Showing the flavonoids determination

Table 4 – Showing the tannin determination

Table 5 – Showing the total phenol determination

Table 6 – Showing the ascorbic acid absorbance

Table 7 – Bioactivity of compounds detected through GC-MS screening of methanolic extract of scent leaves

LIST OF FIGURES

Figxure 1 – Graph showing the flavonoids determination

Figure 2 – Graph showing the tannin determination

Figure 3 – Graph showing the total Phenol determination

Figure 4 – Graph showing the reducing standard curve.

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