PREPARATION OF BUFFER SOLUTION: “TRIS BUFFER SOLUTION”

PREPARATION OF BUFFER SOLUTION: “TRIS BUFFER SOLUTION”

ABSTRACT
Tris buer solution was prepared in the laboratory by dissolving 4.6g of boric acid, 6.5g of EDTA and 60.5g of trisma base in 1000cm3 of distilled water. Comparative study were done on the commercially produced tris buffer solution, using and laboratory produced tris buffer solution, using pH meter for the p H and by carrying out genotype test for the efficacy of Tris buffer solution prepared in the laboratory was used as an electrolyte. This
genotype test reduces the occurrence of sickle cell anemia. This expresses the efficacy
rate and pH of laboratory produced Tris buffer solution as compared with that of commercially produced tris buffer solution. The result obtained for the pH of laboratory produced tris buffer solution was found to be 9.0 while that of commercially produced tris buffer solution was found to be 8.9. the result obtained for the efficacy
using genotype test showed that tris buer solution produced in laboratory has more efficacy
than that produced commercially, but the difference is not significant.

CHAPTER ONE
1. INTRODUCTION
Martin et al (1989) defined buer as a solution that has the ability to resist change in pH, when small amounts of strong acids or bases are added to it. For example, when 0.01 mole of strong acids or base is added to distilled water, the pH drops to 2 with the acids and rises to 12 with the base. If the
same amount of acid or base is added to an acetic acid-sodium acetate buer,
the pH may only change a fraction of a unit. Buers are important in many areas of chemistry. When the pH must be controlled during the course of a reaction, the solutions are one buered.
This is one the case in biochemistry when enzymes or proteins are being studied. Our blood is one buered to a pH of 7.4. Variations of a few
tenths of a pH unit can cause illness or death. Acidosis is the condition when pH drops too low. Alkalosis is the condition when pH becomes higher than normal. Acidosis or alkaloids acts the functions of the heart.

PREPARATION OF BUFFER SOLUTION: “TRIS BUFFER SOLUTION”