NUTRIENT REQUIREMENTS FOR IN VITRO PROPAGATION OF RICINUS COMMUNIS L. ZYGOTIC EMBRYO USING THE BASAL MEDIA OF MURASHIGE AND SKOOG, GAMBORG ET AL. AND SCHENK AND HILDEBRANDT

 NUTRIENT REQUIREMENTS FOR IN VITRO PROPAGATION OF RICINUS COMMUNIS L. ZYGOTIC EMBRYO USING THE BASAL MEDIA OF MURASHIGE AND SKOOG, GAMBORG ET AL. AND SCHENK AND HILDEBRANDT, A RESEARCH PROJECT TOPIC ON PLANT SCIENCE

ABSTRACT

This study was carried out on the nutrient requirements for the in vitropropagation of Ricinus communis employing the basal media of Murashige and Skoog (1962), Gamborg et al. (1968), and Schenk and Hildebrandt (1972) using zygotic embryos as explants. Zygotic embryos were excised from mature seeds and cultured on the three basal media with 3 per cent sucrose and 8 g/l of agar. Plant growth regulators were not added to the media. This study was done to determine the most suitable basal medium for the growth of R. communiszygotic embryo. The results obtained showed that the three basal media employed supported in vitro regeneration of the embryo explants. The highest mean shoot length (4.450±0.231 cm), the highest mean root length (2.190±0.262 cm), highest mean fresh weight (0.365±0.032 g), highest mean leaf area (1.999±0.189 cm²), highest mean per cent sprouting (91.660±0.000), and highest mean number of roots (4.600±0.163) were observed on Murashige and Skoog medium whereas the highest mean sprout rate (0.330±0.000) was obtained on Murashige and Skoog and Gamborg et al. media. The embryo explants were able to develop into normal plantlets even in the absence of growth regulators. This may suggest that endogenous hormones in the zygotic embryo were present at an optimal level to support regeneration. Results from this study indicated that Murashige and Skoog basal medium was the best basal medium for the in vitro propagation of Ricinus communis zygotic embryo. The results are discussed in the light of its potential for mass production of Ricinus communis for its economic values.

CHAPTER ONE

INTRODUCTION

Castor oil plant (Ricinus communis L.) is a non food, drought resistant, energy plant gaining attention for producing biofuel as biodiesel in developing countries (Kamrun, 2013). As an oil bearing biomass feedstock, it can ensure an alternative source of energy and reduce the over dependency on fossil fuel (Kamrun, 2013). Ethiopia is considered to be the most probable site of origin of castor oil plant because of the presence of high diversity (Anjani, 2012). However, according to Sujatha et al. (2008) the plant originated from Abyssinia. It is distributed throughout the tropics and the subtropics, and is well adapted to the temperate region (Sujatha et al., 2008).

 NUTRIENT REQUIREMENTS FOR IN VITRO PROPAGATION OF RICINUS COMMUNIS L. ZYGOTIC EMBRYO USING THE BASAL MEDIA OF MURASHIGE AND SKOOG, GAMBORG ET AL. AND SCHENK AND HILDEBRANDT, A RESEARCH PROJECT TOPIC ON PLANT SCIENCE