ISOLATION AND IDENTIFICATION OF RHIZOPUS FROM DECAYING BREAD

ISOLATION AND IDENTIFICATION OF RHIZOPUS FROM DECAYING BREAD

ABSTRACT

The Isolation and Identification of Rhizopus       from a Decaying Bread were conducted.  The black mould used in the practical was collected from bread.  It is allowed to stay in moist environment or at room temperature for 2 to 3 days. The mould is then inoculated in the nutrient egar (N.A.) incubated and was isolated and view under the microscope for cultural characteristics.

In the experiment a mass of soft, closely woven white, silky threads was observed.  In the microscope examination, non-deptate masses of round black spores, rhizoid.  This characterized by Rhizopus SP.

In conclusion, since foodstuff from which the mould is isolated is bread, Rhizopus SP which is more prominent in bread than in any other foodstuff.

TABLE OF CONTENTS

Title page

Certification

Dedication

Acknowledgement

Abstract

Table of contents

CHAPTER   ONE

INTRODUCTION

1.1     Aim and Objectives

1.2     Hypothesis

1.3     Statement of Problem

1.4     Significance of Study

1.5     Limitation of the Study

CHAPTER   TWO

2.1     Composition of Bread and It’s Requirement

2.2     Source of Contamination

2.3     Rhizopus as a spoilage Organism

2.4     Physiological Characteristic of Rhizopus

2.5     Myeotoxins

2.6     Preservative

CHAPTER   THREE

METHODOLOGY

3.1     Sample Collection

3.2     Direction for Method of preparation of the Medium

3.3     Isolation Method

3.4     Method of Identification

CHAPTER   FOUR

GENERAL MORPHOLOGY

4.1     general Morphology of Rhizopus

4.2     Mode of Life

4.3     Reproduction of Rhizopus

CHPATER   FIVE

RESULT

5.1     Table I

5.2            Table II

CHAPTER   SIX

CONCLUSION AND RECOMMENDATION

6.1     Conclusion

6.2     Recommendation

REFERENCES

CHAPTER   ONE

INTRODUCTION

Rhizopus nigricans belongs to a group of fungi known as the “black moulds”.  It is also known commonly as the “read mould” because if grows freely on moist, state bread.  It appears as a delicate black mass.  It is a common mould, which is widespread in occurrence.  Rhizopus forms a mass of soft, closely woven white silky threads.  This mass, which is known as a mycelium is the vegetative part of the Fungs.  Each silky thread like structure is a hypha.  The ‘mycelium grows not only on the surface of the bread but into it as well.   The bread is known as the substrate.

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