EFFECT OF METAL IONS ON ARGINASE FROM THE KIDNEY OF LOCAL GOAT (CAPRA AEGAGRUS HIRCUS)

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EFFECT OF METAL IONS ON ARGINASE FROM THE KIDNEY OF LOCAL GOAT (Capra aegagrus hircus)

ABSTRACT

This research is aimed at isolating, partially purifying and determination of arginase from the kidney of a local goat. Methods used in the isolation and partial purification of the enzyme include; Ammonium sulphate precipitation, dialysis.etc. Result from this work showed that arginase isolated from a local goat has a specific activity of 0.358 with a yield of 12.12% incubation of the enzyme with different metal ions shows that the enzyme is inhibited by Nacl and Bacl2.

TABLE OF CONTENTS

CHAPTER ONE

1.0     Introduction  and Literature review

1.1     Introduction

1.2     Literature review

1.2.1  History of arginase

1.3     Occurrence and distribution of arginase

1.3.1  Bacteria

1.3.2  Fungi

1.3.3  Lichens

1.3.4  Yeast

1.3.5  Insects

1.4     The substrate: Arginase

1.5     Scientific classification of local goat

1.6     Aim and Objectives

CHAPTER TWO

2.0     Materials and methods

2.1     Materials

2.1.1  Apparatus used

2.2     Methods

2.2.1  Assay

2.3.2  Protein determination

2.3.3  Purification procedure

CHAPTER THREE

3.0     Results

3.1     Purification and Homogeneity

3.2     Effect of metal ion on arginase from kidney of a local goat

CHAPTER FOUR

5.0     Discussion, Conclusion and Recommendation

5.1     Discussion

5.2     Conclusion

5.3     Recommendations

References.

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