ANTIBACTERIAL EFFECT OF NEEM TREE (AZADIRACHTA INDICA) LEAF EXRACT ON STAPHYLOCOCCUS AUREEUS

ANTIBACTERIAL EFFECT OF NEEM TREE (Azadirachta indica) LEAF EXRACT ON STAPHYLOCOCCUS AUREEUS (SCIENCE PROJECT TOPICS AND MATERIALS)

ABSTRACT

The main focus of this study was to determine the antibacterial effect of Neem tree (Azadirachta indica) leaf extract on Staphylococcus aureus. The Neem tree leaf extract was tested in terms of the different levels of concentration with (a) 25% concentration, (b) 50% concentration, (c) 75% concentration, (d) 100% concentration on Staphylococcus aureus. It specifically compared the significant differences of the different levels of concentration of Neem tree leaf extract, and also compared to the positive control. The plant materials were collected and brought to the West Visayas State University Biological Science Laboratory where the experimentation was conducted.

Completely Randomized Design (CRD) was the research design used in the study. The interpretation of the results was based on the Claustro et.al zone of inhibition scale. The statistical analysis (One- way ANOVA) was not employed due to the reason that the zone of inhibition is zero.

Results of the study revealed that there was no significant differences on the effect of the different levels of Neem tree (Azadirachta indica) leaf extract on Staphylococcus aureus.

 INTRODUCTION OF THE STUDY

1.0 BACKGROUND OF THE STUDY   

Philippines is a country that has plenty of resources and biodiversity. Plants in different species just sprout and grow anywhere.Vegetations remain to be the most important part of human existence.

Medicinal substances derived from different plants around us have become the most sought sources of medicines. However, a considerable number of people in our generation still do not recognize the value and significance of medicinal plants.  Many people continue to rely on synthetic drugs for treatment of common illnesses despite their being expensive.

Basically, the researchers had initiated a study that may provide appropriate answers tosome common health problems experienced by human beings.

Nowadays, skin problems also proliferate fast and are equally disturbing just like any other diseasesaffecting our communities.  This is the primary reason why researchers conceptualized this study to determine the anti-bacterial components of Neem (Azadirachta indica) leaves extract and its possible effects against Staphylococcus aureus, the bacteria that concerns most of the common skin problems.

Neem is a tropical tree that has been declared non-toxic to humans. Previous studies revealed that it contains anti-bacterial properties and high-content of anti-oxidant called polyphenols. Neem tree extracts has an endodontic anti-microbial agent, an alternative to frequently employed Sodium Hypochlorite and Chlorhexide; both to inhibit the growth of endodontic pathogens.

Sodium Hypochlorite is now used in endodontic during root canal treatments. It is the medicament of choice due to its efficacy against pathogenic organisms and pulp digestion. Chlorexide is a chemical antiseptic. It is effective on Gram-positive and Gram-negative bacteria.

ANTIBACTERIAL EFFECT OF NEEM TREE (Azadirachta indica) LEAF EXRACT ON STAPHYLOCOCCUS AUREEUS (SCIENCE PROJECT TOPICS AND MATERIALS)