A HISTOLOGICAL AND MORPHOMETRIC ASSESSMENT OF ENDOCRINE AND DUCTULAR PROLIFERATION IN THE ADULT RAT PANCREAS USING AN OCCLUSIVE PANCREATIC DUCT LIGATION MODEL

There is an urgent need to develop research capacity at UNW. To this end the Departments of Nursing Science, Biology and Agriculture and involved to a lesser or greater degree in planning, implementing and logistical support for the development of an Experimental Biology Facility. After consultation with interested parties and promoters, it was decided to develop facilities that can be expanded. Hence this project of studying endocrine tissue regeneration in laboratory rats. Not only is the topic contentious but attainable within the limited budget available. MATERIALS STAGE 1 *items acquired on own budget *Experimental animals Breeding pairs from MRC Animal housing facilities University of the North-West (UNW)Department of Biology *Micro-dissecting kit Carolina Biological Supply Co @$75-00 *Tissue forceps 4″ straight 62-4590 @ $6-50 ea *Rochester Pean Hemostatic Forceps 62″ 62-5321 @ $6-74 ea *Mayo Hegar Needle holder 62-5500 @ $7-15 ea *Saggital (Maybaker) UNW *Penicillin & Streptomycin UNW *Suture material (taper-cut needles) UNW *Surgical gloves UNW Microscope with camera and dissection microscope UNW Ether, Bouin’s fixative, ethanol, xylene/xylol UNW *Lymph-angiocaths for penetration of tail vein Microtome UNW Paraplast paraffm wax and heating facilities UNW Tissue-Tek moulds MRC *Microscope slides 63-2100 @ $7-30/box X 7 *Cover slips 25 mm 63-3017 @ $11-95/100 X 3 Stellenbosch University